Previous studies used an automatic imaging and scoring platform to quantify and analyze tumor-infiltrated CD8+ T lymphocytes, CD133+ CSCs, and TB within the TME to achieve a greater level of standardization than subjective, manual reporting11,22,23; however, these cells have yet to be studied together in patients with PDAC in an ‘anti-/pro-tumor model’ using an automatic quantification and analysis approach. The gene discussed is PROM1; the disease is neoplasm.