IL1B and classic lissencephaly: Furthermore, the western blot analysis was performed to determine the protein expression levels of iNOS, IL-1β, and TNF-α, which were strongly upregulated in the control group compared to those of the normal group, whereas these genes were dose-dependently suppressed in the OS groups, and the iNOS and IL-1β levels were dramatically suppressed in all doses in the ILS groups.