Although PD-L1 blockade or CD4+ T cell depletion alone had no impact on tumor vessel perfusion compared with control group, concurrent PD-L1 blockade and CD4+ T cell depletion significantly improved tumor blood vessel perfusion compared with all the other groups in the MCA38 colorectal tumor model (Figures 5(b) and 5(c) and Supplementary Figure S12). The gene discussed is CD274; the disease is colorectal neoplasm.