In the tumor-draining lymph nodes, CD4+ T cell depletion or its combination with PD-L1 blockade therapy significantly elevated the proportions of CD44+CD69+CD8+, as well as central memory CD44+CD62L+CD8+ and effector memory CD44+CD62L−CD8+ T cells (Figure 3 and Supplementary Figure S10, S11). This evidence concerns the gene CD8A and neoplasm.