In the present study, the levels of LZM, ACP and AKP in vaccinated groups were up-regulated, which was similar to the results in C. carpio with A. hydrophila Aha1 vaccine immunization and other previous studies, indicating that both OmpA subunit vaccine and inactivated vaccine can improve host non-specific immunity and enhance their ability to resist infection (10, 36). The gene discussed is NDUFAB1; the disease is infection.