Figure 4A shows seven types of immune cells from PBMC, as expected. The percentage of monocytes was increased in rejection groups except CAN group, while in Late_ACR group, the CD4+ T cells and CD8+ T cells were also elevated (p < 0.05). Four PCA plots indicated more robust clusters from deconvolution gene expression levels of monocytes than bulk RNAseq between four types of rejection and control group (Figure 4B). Meanwhile, the 20 differential genes in monocytes also showed more significant and more relevant differential expressions analyzed by heatmaps (Figure 4C). The gene discussed is CD4; the disease is Crouzon syndrome-acanthosis nigricans syndrome.