Treatment with anti-CLDN18.2-anti-CD28 also produced more interferon-γ (IFN-γ) and tumour necrosis factor-α (TNF-α) in splenocytes and reduced the levels of immunosuppressive cells, including tumour-associated macrophages and myeloid-derived suppressor cells, in tumour tissues. The gene discussed is CLDN18; the disease is neoplasm.