Then, multivariate Cox analysis showed that Ki-67(HR=1.014, 95%CI,1.007-1.020, P<0.001), tumor size(HR=1.056, 95%CI,1.021-1.092, P=0.001), MVI(HR=1.503, 95%CI,1.114-2.028, P<0.01) and the PA-TACE timing (HR=1.515, 95%CI,1.139-2.015, P<0.01) were independent prognostic factors for RFS in HCC patients. The gene discussed is MKI67; the disease is hepatocellular carcinoma.