APC and malignant colon neoplasm: In our work, we employed RKO and SW480 or SW620 human colon cancer cell lines as representative of normal Wnt signaling (RKO, which expresses normal APC protein) and constitutively active Wnt signaling (SW480 and SW620, which express a truncated version of APC and representative of distinct stage of progression in the same patient) (17).