Furthermore, although EGR1 and AChE levels did not vary significantly between sexes, they were both significantly negatively correlated with soluble Aβ(1–42) in pooled 3xTg-AD males but not females, which indicates that the soluble Aβ(1–42) has an inhibitory effect on the expression of EGR1 and AChE, while higher estrogens in female mice may abolish such an effect. Here, ACHE is linked to Alzheimer disease.