Since we have previously found reduced EGR1 and AChE in the PFC of 3xTg-AD mice compared with WT mice, especially from the later age group (7–8 months) (Hu et al., 2019), the role of Aβ on the expression of EGR1 and AChE may be brain area-dependent. This evidence concerns the gene EGR1 and Alzheimer disease.