A probability curve was constructed for the combination of CD109+ and EGFR+ tumor-derived EVs based on binary logistic regression (Logit (<b12>p = NPC) = −13.348 + 0.002 CD109 + 0.004 EGFR), with an AUC of 0.934, a sensitivity of 84.1%, and a specificity of 85% for NPC versus healthy subjects (Jia et al., 2016; Sakakura et al., 2016; Sunagawa et al., 2016; Mii et al., 2019; Li H. et al., 2021). Here, CD109 is linked to nasopharyngeal carcinoma.