In this study, we plan to address: (a) the role of MKRN2 in male infertility; (b) whether MKRN2 regulates the expression level of STAT1; and (c) how MKRN2 induces the expression levels of SIX4 and TNC by the transcription factor EBF transcription factor 2 (EBF2). This evidence concerns the gene SIX4 and male infertility.