In our study, it was found that the NMT1 activated the PI3K/AKT/mTOR pathway in GC cells and partially counteracted the inhibitory functions of silenced SPI1 on the PI3K/AKT/mTOR pathway, implying that the activated signaling pathway was implicated in the regulatory effects of NMT1 on GC. Here, SPI1 is linked to gastric cancer.