Finally, we detected ZNF582 protein expression in several ccRCC cell lines, and results also proved that ZNF582 is generally low expressed in ccRCC cells (OSRC2, 786-O, Caki-1, 769-P and A498) compared with the normal human renal tubular epithelial cell line HK2 (Fig. 1C). Here, ZNF582 is linked to nonpapillary renal cell carcinoma.