ZNF334 and neoplasm: Next, to evaluate the role of the targeted demethylation of ZNF334 on tumor growth in vivo, 5 × 106 HCT116 cells transfected with the dCas9-multiGCN4/scFv-TET1CD-sgZNF334 or the dCas9-multiGCN4/scFv-TET1CD-sgRNA were injected into the flank of female wild-type (WT) BALB/c nude mice to establish a subcutaneous tumor model (Fig. 5A).