OLFM4 and esophageal squamous cell carcinoma: We applied the NMF algorithm to analyze the mutational signatures of the Fudan cohort and other ESCC cohorts, including the TCGA cohort, Moody’s cohort, etc. The integrated findings revealed that the SBS16 signature, which was associated with the ESCC patients with a drinking habit and OLFM4 mutation exhibited positive impacts on CDKs activation and thus enhancing DNA replication evidenced by the related markers, indicating the potential medicative of Dinaciclib for drinking ESCC patients.