Interestingly, the amplification of TP63 at chr3q28, prominent in the IEN phase, showed positive impacts on DNA replication, implying the combined effects of Ca2+ signal and TP63 amplification on DNA replication in the IEN phase and further indicating the functions of chr3q gain in the transmit process from the NT phase to the IEN phase and on DNA replication in ESCC progression. This evidence concerns the gene TP63 and esophageal squamous cell carcinoma.