The results revealed the association of ERK1/2, CDKs, and GSK-3 with the motif of PGK1 (sP)53, whose downstream substrates and the related corresponding phosphorylations, including MAP1S (S759), FOXK1 (S420), MAP2K1 (T386), etc., were increased in ESCC progression at the protein and phosphoprotein levels (Kruskal–Wallis test, FDR < 0.05, stage 9 vs. stage 1 ratio ≥ 2) (Fig. 7f). The gene discussed is MAP2K1; the disease is esophageal squamous cell carcinoma.