Of note, the incorporation of the proteins in Wnt signaling affected by MACF1 mutation, were highly expressed in the A-ESCC phase (Kruskal–Wallis test, FDR < 0.05, A-ESCC vs. NT ratio ≥ 2), such as GSK3A, GSK3B, CSNK1A, CSNK2B, DVL3, etc. (Fig. 3i). The gene discussed is MACF1; the disease is esophageal squamous cell carcinoma.