At the phosphoprotein level, we also detected the increased phosphorylation of glycolysis (e.g., ENO1 S272, PGAM1 S189, etc.)in ESCC progression (Kruskal–Wallis test, FDR < 0.05, A-ESCC vs. NT ratio ≥ 2) (Supplementary Fig. 6o). This evidence concerns the gene ENO1 and esophageal squamous cell carcinoma.