The only identified motif (sP) of PGK1 was ubiquitously (125/145) detected, and the expression of PGK1 S203 was also gradually increased with ESCC progression (Kruskal–Wallis test, FDR = 0.011, stage 9 vs. stage 1 ratio = 2.44) (Fig. 7e and Supplementary Fig. 8b). The gene discussed is PGK1; the disease is esophageal squamous cell carcinoma.