In addition, we found that PGK1 and the phosphorylation (S203) were overrepresented in the kinases highly expressed group (two-sided Wilcoxon rank-sum test, FDR < 0.05, highly vs. lowly expressed group ratio ≥ 2), especially in ERK2 and CDK2 highly expressed group (Fig. 7g), indicating the potential functions of ERK2 and CDK2 in the activation of PGK1 (S203) in ESCC progression. This evidence concerns the gene MAPK1 and esophageal squamous cell carcinoma.