The developed PDL-1 functionalized Cur-loaded albumin-NPs (P-HAS/Cur-NPs) were evaluated for cell uptake efficiency and dose dependent (12.5, 25, 35, and 50 μM) cytotoxicity against the BC cells (MCF-7 and MDA-MB-231) at different time periods (24, 48, and 72 h). The gene discussed is CD274; the disease is breast cancer.