HDGFL2 and infection: An alternative explanation may be the decreased sensitivity of the Pf-LDH RDT compared with the HRP2-only RDT for parasite densities ≤1000 parasites/μL, and the shorter half-life of Pf-LDH, or the trend for HRP2-negative parasites to circulate as low density (asymptomatic) infections, potentially increasing the multiplicity of infection.