EGFR and laryngotracheoesophageal cleft: Using molecular weight cut-off (MWCO) filtration, we confirmed that concentrated secreted proteins of >30 kDa from targeted inhibitor-treated cells, their respective DR clones, and EGFR-TKI-treated LC patients display distinctively enriched core fucosylation and core α–1,6-linkages-containing N-glycans, but less so in >100 kDa pooled proteins (Figure 1—figure supplement 10C and D).