The expression of CD47 was assessed in de-identified osteosarcoma specimens from eight chemotherapy-naïve human patients, one de-identified osteoma specimen, one de-identified normal bone specimen (Cooperative Human Tissue Network), three human osteosarcoma cell lines (U-2 OS, Saos-2, and MNNG/HOS) and a normal bone cell line (hFOB 1.19) by qPCR, using glyceraldehyde 3-phosphate dehydrogenase (GAPDH) as a control marker [50]. The gene discussed is GAPDH; the disease is osteoma.