CTCF and cancer: The HiC-Bench method uses a 2D-fused lasso as a machine learning method to reproduce the Hi-C contact matrix, and then TAD boundaries can be classified based on their insulator scores. The results indicate that the higher the insulator score of the TAD boundary, the higher the CTCF level and that the score may vary among cell types. Furthermore, it was found that SEs are preferentially insulated by strong boundaries and that strong TAD boundaries and SE elements frequently co-occur in cancer patients.