Then, we detected the SPON2 mRNA expression levels in TNBC cells MDA-MB-231, non-TNBC breast cancer cells MCF-7, and normal breast cells MCF-10A by qPCR, which showed that SPON2 expression was higher in MDA-MB-231 than MCF-7 and higher in MDA-MB-231 than MCF-10A, Figure 1D. The gene discussed is SPON2; the disease is breast cancer.