Since it has been well established that cancer cells develop radioresistance by activating PRMT5-mediated upregulation of HR & NHEJ proteins upon IR treatment, plausibly we then investigated mRNA transcription of HR & NHEJ proteins in A549 & U87MG cells (13) In both A549 & U87MG cells, mRNA fold changes depicted that JNJ-64619178+IR treated cells significantly downregulated the transcription of HR (RAD51, RAD51D, RAD51AP1, BRCA1, BRCA2) & NHEJ (NHEJ1) proteins compared to DMSO+IR treated cells (Figures 4C, F). This evidence concerns the gene PRMT5 and cancer.