To assess γH2AX foci, LNCaP (AR+) and DU145 (AR-) prostate cancer cells were treated with JNJ-64619178 for 4 days, then the cells were exposed to 2 Gy of X-ray (IR+) for inducing DSBs, next the cells were incubated for 6hrs to let them employ repair proteins at the site of DSBs (22). Here, AR is linked to Familial prostate cancer.