To explore the therapeutic significance of PES1 in ICB therapy in vivo, we inoculated SCR and shPES1 AKR cells into C57BL/6 mice, and anti-PD-1 antibody (InVivoMAb anti-mouse PD-1 (CD279), BE0273, BioXcell) was injected on days 7, 10 and 13 after tumor cell inoculation. The gene discussed is PDCD1; the disease is neoplasm.