Based on the results of DEG3 and ROC curves in GSE66724 and GSE58294 (Fig 7D and 7E), we filtered eight hub genes of AF-related stroke (AUC > 0.8), including EIF4E3, ZNF595, ZNF700, MATR3, ACKR4, ANXA3, SEPSECS-AS1, and RNF166. This evidence concerns the gene SEPSECS and atrial fibrillation.