Since IL-1β was involved in leukocyte chemotaxis in GO biological terms, played a main role in interacting with DEGs as shown by protein interaction analysis (Figure 4e), and its production was not affected by neutrophil accumulation (Figure 6g), we then hypothesized to examine whether IL-1β is implicated in regulating neutrophil recruitment and intestinal inflammation during colitis. Here, IL1B is linked to colitis.