TLN1 and colitis: Talin-1 has been implicated in the formation of attaching and effacing lesions in response to EPEC and C. rodentium, but this has not been directly studied in vivo.20,21 To evaluate the role of talin-1 in epithelial cells during pathogenic colitis, we used Tln1Δepi and littermate control Tln1fl/fl mice.22,24 We first confirmed knockdown of Tln1 mRNA (Figure 1a) and talin-1 protein expression in isolated CECs (Figure 1b,c).