In the current study, Carbonic anhydrase inhibitory effect of 20 novel synthesised compounds (3a-t) was tested under in vitro condition against two cystolic (hCA I and II) and membrane bound tumour related isoforms i.e., hCA IX and XII, along with the standard inhibitor acetazolamide (AAZ) in a stopped-flow CO2 hydrase assay.36 The gene discussed is CYP24A1; the disease is neoplasm.