To further verify the heterogeneity of IFIT1 and PARM1 in normal and osteosarcoma tissues, we cultured the osteoblast cell line hfob 1.19 and the osteosarcoma cell line saos-2, and detected the expression of IFIT1 and PARM1 by RT-qPCR, and the results showed that compared with osteoblasts, osteosarcoma cells had high expression of IFIT1 (Figure 5A), PARM1 was lowly expressed (Figure 5B). Here, IFIT1 is linked to osteosarcoma.