In the high-risk group, signaling pathways or biological processes such as cell junction organization, DNA packaging complex, integrin binding and focal adhesion were enriched, while in the low-risk group, cellular respiration, ribosome, NADH dehydrogenase activity, oxidative phosphorylation and other signaling pathways Enrichment (Figures 5A–J), in order to illustrate the effect of mutations on the development of COAD, the APC mutation rate of our high-risk patients was lower than that of low-risk patients, and APC mutations were mostly enriched in low-risk patients (Figures 5K–L). This evidence concerns the gene ENSG00000251605 and colon adenocarcinoma.