As anticipated, there were upregulated protein levels of total APP and ? secretase (PS1) in the hippocampal tissues of 3×Tg-AD mice, while the levels of ß secretase (BACE1) were slightly increased and no significant change in a secretase (ADAM 10) compared with WT mice (Figures 6A, B). This evidence concerns the gene BACE1 and Alzheimer disease.