ZBTB4 and neoplasm: The current study demonstrated that TMB was strongly correlated with the efficacy response to immunotherapy, and we computed their Spearman correlation in each tumor and observed that ZBTB4 expression was significantly negatively correlated with TMB in 17 tumors (Fig 4B), including GBMLGG, LGG, LUAD, BRCA, ESCA, STES, STAD, PRAD, HNSC, KIRC, LUSC, LIHC, THCA, PAAD, UVM, BLCA and DLBC.