It was previously shown that dysregulated hypomethylation of the NANOG promoter is observed in the CD133 (+) population of human HCC cell lines.[35] Similar to these CD133 (+) cell lines, we observed primary TICs were hypomethylated in the Nanog promoter proximal region, consistent with the observed higher expression levels of NANOG (Figure S7, Supporting Information)). The gene discussed is PROM1; the disease is hepatocellular carcinoma.