Western blot, immunofluorescence and FACS quantification showed that EPCAM− cells presented a significant reduction in γ-H2AX compared with EPCAM+ and EPCAM−Rhoj-KO tumour cells after cisplatin/5FU administration (Fig. 4b,c and Extended Data Fig. 6d,e), supporting the notion that RHOJ prevents accumulation of DNA damage in EMT tumour cells. The gene discussed is RHOJ; the disease is neoplasm.