Western blot, immunofluorescence and FACS quantification showed that EPCAM− cells presented a significant reduction in γ-H2AX compared with EPCAM+ and EPCAM−Rhoj-KO tumour cells after cisplatin/5FU administration (Fig. 4b,c and Extended Data Fig. 6d,e), supporting the notion that RHOJ prevents accumulation of DNA damage in EMT tumour cells. This evidence concerns the gene H2AX and neoplasm.