To assess the physiological relevance of our findings we treated HT29 and PANC1 cancer cells with Tunicamycin and PNGase-F (Fig. 3B) which, as in HEK293T-NTSR1 cells, induced a strong increase of the NTSR1-LP form, while the NTSR1-high form was undetectable. This evidence concerns the gene NGLY1 and cancer.