However, recent studies show that while prolonged exposure to high-fat high-sugar diets leads to morbid obesity and NAFLD, changes in glucose homeostasis can be highly dynamic, with glucose intolerance and insulin sensitivity initially deteriorating but being indistinguishable to that of chow mice following > 24 weeks of high-fat feeding30,31, as is the case in our study. Here, INS is linked to morbid obesity.