Spautin-1 almost completely inhibited the ability of USP10 to deubiquitinate RUNX1 in U251 and GBM1 cells (Fig. 6D), while this inhibitor reduced the expression of RUNX1 and MES subtype markers and increased the expression of PN subtype markers in GBM cells. This evidence concerns the gene RUNX1 and glioblastoma.