Although we observed 5AZADC-induced upregulation of MHC class I in both B16-ISG and B16-ISG-STINGKO cell lines (Supplementary Fig. 3c, d), unlike 5AZADC-pretreated B16-ISG cells, further stimulation with the STING agonist did not increase surface expression of MHC class I in 5AZADC-pretreated B16-ISG-STINGKO cells, suggesting that DNA demethylation-induced increased IFN-β production and upregulation of MHC I in response to agonist stimulation in melanoma cells is STING mediated. Here, IFNB1 is linked to melanoma.