In this work, to identify tumor intrinsic kinase that contributes to the evasion of CD8 T cells in an unbiased fashion, we perform an in vivo kinome knockout (KO) CRISPR screen evaluating 713 kinases in glioma cells intracranially implanted into wild-type (WT) and CD8 KO mice. The gene discussed is CD8A; the disease is central nervous system cancer.