Since podocyte injury and loss is a hallmark of DN (Ma, Chen, et al., 2019; Tao, Chaudhari, et al., 2022; Tao, Mallet, et al., 2022), we furthermore examined the number of podocytes by staining WT1 in kidney sections from male and female diabetic and control mice. The gene discussed is WT1; the disease is liver dysplastic nodule.