Specifically, we demonstrate that physiologically relevant stimulation of TRPV4 expression and activity in cystic cells by high KCl diet increases basal [Ca2+]i levels, partially restores mechanosensitivity, and causes intracellular AQP2 retention (reduced cAMP levels) to markedly slow the development and growth of renal cysts in PCK453 rats (Figure 8, right panel). This evidence concerns the gene TRPV4 and Renal cyst.