Given the previous evidence showing that the inactivation or inhibition of PP2A promotes cancer development84–86, our experimental validation of the disrupted PPP2R1A subnetwork (Fig. 6g) caused by the PPP2R1A p.Arg258Cys mutation underscores the value of NetFlow3D in identifying novel cancer driver mutations and illuminating potential tumorigenic mechanisms. Here, PTPA is linked to cancer.