Next, we found that ALDOC and ENO2 knock down, either as a single entity or in combination, attenuated the spheroids forming ability in both cell lines regardless of the culture media conditions as shown by the markedly reduced cell viability (Fig. 6B) and tumor spheroids size (see images and relative histograms in Fig. 6C) (*p-value < 0.05, **p-value < 0.01, ***p-value < 0.001). This evidence concerns the gene ENO2 and neoplasm.