We collected the resected carcinoma tissues from CRC patients and conducted immunohistochemical (IHC) staining analysis to examine the expression of some representative genes including NEK8, CHRM3, ANO7, B3GNT6, NEURL1, ODC1 and KCNMA1 in colon normal tissue, adenoma tissue and carcinoma tissues. This evidence concerns the gene NEK8 and adenoma.