We collected the resected carcinoma tissues from CRC patients and conducted immunohistochemical (IHC) staining analysis to examine the expression of some representative genes including NEK8, CHRM3, ANO7, B3GNT6, NEURL1, ODC1 and KCNMA1 in colon normal tissue, adenoma tissue and carcinoma tissues. The gene discussed is NEK8; the disease is colorectal carcinoma.