In contrary, repression of GPC1 gene expression in T24 bladder carcinoma, HepG2 liver hepatocellular carcinoma and U87 brain glioma cells using CRSPR/Cas9 or siRNA resulted in significant attenuation of cancer cell proliferation indicating the potential of GPC1 as target for future cancer therapies. The gene discussed is GPC1; the disease is brain glioma.