Specifically, we knocked-out exon 1 of GPC1 with CRISPR/Cas9GPC1 and transiently interfered with GPC1 transcript using siRNAGPC1 in cancer cells from patients with urinary bladder carcinoma (T24 cells), malignant brain glioma (U87 cells) and liver hepatocellular carcinoma (HepG2 cells). This evidence concerns the gene GPC1 and brain glioma.