To evaluate the changes in glucokinase expression in human islets during T2D progression, we collected human pancreatic sections from 11 nondiabetic (ND, FBG: [4.50–5.47] mM), 10 impaired fasting glucose (IFG, FBG: [5.66–6.80] mM), 9 wT2D (FBG: [5.00–7.36] mM), and 5 uT2D (FBG: [8.15–13.7] mM) subjects. The gene discussed is GCK; the disease is type 2 diabetes mellitus.