By using the RV specimen, we performed the Western blot analysis and the result demonstrated that the protein expression of ß‐MHC, an indicator of cardiac hypertrophy, the protein expression of BNP, an indicator of pressure‐overload/heart failure biomarker, protein expressions of NOX‐1 and NOX‐2, two indices of oxidative stress and γ‐H2AX, an indicator of DNA‐damaged marker, were significantly increased in MCT group than in the SC and MCT‐PTU groups and significantly increased in MCT‐PTU group than in that of the SC group (Figure 10). Here, NPPB is linked to cardiac hypertrophy.