CD69 and neoplasm: There was no significant difference in the expression of CD25 and CD69 on the surface of B7-H3-CAR-Jurkat and Jurkat cells after co-incubation with tumour cells with a low expression of B7-H3 (Fig. 2C, D) CD69 is a marker for an early activation of T lymphocytes while CD25 is for late activation of T lymphocytes; both CD25 and CD69 are involved in the T lymphocytes proliferation, and their expression is correlated with the degree of immune response [46, 47].