In addition, serum ALT, AST and ALP activities (Fig. 4e), the Hyp content (Fig. 4f), α-SMA expression (Fig. 4d, g) (Full-length blot of α-SMA is presented in Additional file 2: Fig. 9) and the mRNA expression levels of proteins related to liver fibrosis, including TNF-α, TGF-β1 and Col(1) (Fig. 4h), were increased significantly in the BM-MSCNumb-KD group compared to the BM-MSCKD-EV group (P < 0.05 or P < 0.01). This evidence concerns the gene GPT and Hepatic fibrosis.