To address whether dual reactivity to DNase1L3 and dsDNA is exclusive of autoantibodies derived from autoreactive VH4-34+ B cells or is a common feature among anti-dsDNA antibodies, we additionally analyzed four SLE-derived non-VH4-34 IgG monoclonal antibodies originally defined as anti-dsDNA from which both the IgH and IgL variable gene sequences are publicly available (i.e., 32.B9, 33.H11, 33.C9, and RH-14)50,51. Here, DNASE1L3 is linked to systemic lupus erythematosus.