IHC assays showed that C.B CM + 5-FU group have lower expression level of Ki67 and higher expression level of CASPASE 3, lower MYC and TYMS when compared with 5-FU alone (Figure 4d), indicating that C.B’s negative impact on MYC-TYMS axis can be recapitulated in mouse xenograft CRC model. This evidence concerns the gene MKI67 and colorectal carcinoma.